The structural alterations of mitochondria in ONO-AE-248-induced non-apoptotic programmed cell death of neutrophils
- VernacularTitle:ONO-AE-248诱导中性粒细胞非凋亡性程序化死亡线粒体形态结构的变化
- Author:
Jian YANG
;
Jiajia LIU
;
Xiaojie XUE
;
Zi ZHANG
;
Hao HE
- Publication Type:Journal Article
- Keywords:
Mitochondria;
Neutrophils;
ONO-AE-248;
Apoptosis;
Non-apoptotic programmed cell death
- From:
Chinese Journal of Immunology
1985;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the structural alterations of mitochondria and its role in neutrophil death induced by ONO-AE-248.Methods:Human neutrophils were cultured in vitro with ONO-AE-248(5?10-5 mol/L)and medium alone. Transmission electron microscopy(TEM) was used to detect the structural alterations of mitochondria and the level of mitochondria membrane potential by flow cytometry using mitocapture dying.Results:ONO-AE-248 resulted in extremely swollen mitochondria within 6 hours. Meanwhile, a rapid loss of mitochondrial membrane potential of neutrophils occurred, especially in 3 hours and 6 hours. There were obviously differences between spontaneous apoptosis and non-apoptosis programmed cell death induced by ONO-AE-248.Conclusion:The experiment results suggest that changes of mitochondrial structure and function be typically morphological, physiological and biochemical features in this unique form of neutrophil death, and that the mitochondrial pathway might play a more important role in ONO-AE-248-induced death of neutrophils.
