Determination of Methylisothiazolinone and Chloro-methylisothiazolinone in Cosmetics by High Performance Liquid Chromatography
- VernacularTitle:化妆品中甲基异噻唑啉酮及其氯代物的高效液相色谱测定法
- Author:
Chao WANG
;
Qing ZHANG
;
Xing WANG
- Publication Type:Journal Article
- Keywords:
Cosmetics;
High performance liquid chromatography;
Methylisothiazolinone;
Chloromethyl-lisothiazolinone
- From:
Journal of Environment and Health
1989;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop a high performance liquid chromatography method for the determination of methylisothiazolinones and chloro-methylisothiazolinoe in cosmetics. Methods The cosmetic samples were extracted with methanol. The analytes were separated with the mobile phase of V(Methanol)∶V(Water)= 25∶75 at the flow rate of 1.0 ml/min and detected at the wavelength of 276 nm. Results The linear range of the method was 0.05-100 mg/ml for methylisothiazolinone and 0.0125-25 mg/ml for chloro-methylisothiazolinoe. The limit of detection of methylisothiazolinone and chloro-methylisothiazolinoe were 0.08 and 0.2 ?g respectively. The recovery rates were 84.5%-93.9% with the RSD of 2.5%-9.4% (n=6) for methylisothiazolinone and 88.3%-92.9% with the RSD of 2.9%-5.8% (n=6) for chloro-methylisothiazolinoe. Conclusion The method is simple,rapid,accurate and is suitable for the determination of methylisothiazolinone and chloro-methylisothiazolinoe in cosmetics.
