Promoter hypermethylation and transcription level of BLU in bladder cancer
- VernacularTitle:膀胱癌中BLU基因启动子高甲基化和转录水平
- Author:
Jianying ZHANG
;
Zhi LI
;
Yongchun YU
;
Jianmin DUAN
- Publication Type:Journal Article
- Keywords:
bladder cancer;
BLU;
methylation;
transcription;
methylation specific PCR(MSP)
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
1981;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the promoter methylation status and the transcription level of BLU in bladder carcinoma and their significance.Methods The methylation status of promoter and the transcription level of BLU were examined by methylation specific PCR(MSP) and RT-PCR in bladder cancer tissues from 54 patients,adjacent normal tissues from 45 cases and normal bladder tissues from 3 noncancerous patients.Results ① 31.5%(17/54) bladder cancer tissues showed BLU hypermethylation;neither the paired adjacent normal tissues nor the three normal bladder tissues showed BLU hypermethylation.② There was no significant correlation between the methylation status and the clinical pathological parameters in bladder cancer.③ The expression of BLU was absent in 42.2%(19/45) of bladder carcinoma from cystectomy.④ Loss of BLU expression was associated with the stage and grade of the tumor(P
