Experimental Study of Inhibiting Effect of TIMP-2 Gene Transfected by Adenovirus on Extracellular Matrix of Rat Abdominal Aorta
- VernacularTitle:腺病毒介导TIMP-2基因转染抑制大鼠腹主动脉细胞外基质降解的实验研究
- Author:
Xin ZHAO
;
Peirui ZHANG
;
Xianghai YU
;
Mingfang LIAO
;
Suzhen ZHANG
;
Zaiping JING
- Publication Type:Journal Article
- Keywords:
Tissue inhibitor of matrix metalloproteinase Adenovirus vector Gene therapy Rat model
- From:
Chinese Journal of Bases and Clinics in General Surgery
2003;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of TIMP-2 gene that was transfected by adenovirus on extracellular matrix of abdominal aortic through assessing the changes of morphology and histopathology of the rat models with abdominal aortic aneurysm. Methods The rat models with abdominal aortic aneurysm were constructed by intraluminally perfusing porcine pancreatic elastase. Twenty-four SD rats with aneurysm were then randomly divided into 3 groups: AdTIMP-2 group (perfused locally with solution of TIMP-2 gene transfected by adenovirus vector to abdominal aorta), AdCMV group (transfected by non-viral vector), and PBS group. After 14 days, the concentrations of elastin and collagen that were collected from the samples of aortic wall were measured by image analysis system and the fixed aortic tissues were examined by light microscopy and some other specific staining methods. Results None of abdominal aortic aneurysm developed in TIMP-2 gene transfected group, with significantly higher rates of developed aneurysm in the other groups (P
