Determination of Paraquat in Serum by Ultraviolet Spectrophotometry
- VernacularTitle:血中百草枯的紫外分光光度测定法
- Author:
Yanyan ZHAO
;
Huifang LIU
;
Lina HAO
- Publication Type:Journal Article
- Keywords:
Pesticide;
Spectrophotometry;
Paraquat;
Plasma drug concentration;
Hemoperfusion
- From:
Journal of Environment and Health
1992;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method for determining paraquat in the serum by ultraviolet spectrophotometry and present a quantitative index for paraquat poisoning salvage. Methods The sample was deproteinizated by 20% trichloracetic acid(TAC). 50 ?l microcell was used, the detection wavelength was 257 nm. Results The linearity was within 0.05~50 ?g/ml, r=0.999 9. The average recovery rates were 90.0%-102.4% and RSD=3.9%(n=4), the lowest detection limit was 0.01 ?g/ml. Conclusion The method is simple, rapid, precise and suitable for the determination of paraquat in the serum.