Changes of c-jun expression in temporalis muscles caused by hypergravity in Rhesus macaque
- VernacularTitle:高+Gx环境对猴颞肌c-jun蛋白表达影响的研究
- Author:
Chuhua TANG
;
Shenggen SHI
;
Jianzhong ZHANG
- Publication Type:Journal Article
- Keywords:
Hypergravity;
Temporalis muscles;
C-jun expression
- From:
Journal of Practical Stomatology
2001;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the changes of c-jun expression in the monkey's temporalis muscles induced by stress reaction under +Gx loads.Methods:Nine male Rhesus macaques were randomly divided into four groups according to Gx loads and its lasting time:control group was exposed to +1 Gx/300 s overloads(n=2),and experimental groups 1(n=2),2(n=2)and 3(n=3)were exposed to +15 Gx/200 s,+21 Gx/165 s or +21 Gx/140 s respectively.Temporalis muscles tissue was fixed with 40 g/L buffered formaldehyde and embedded in paraffin.Histopathological changes were observed under light microscope.The expression of c-jun was detected by immunohistochemical PicTureTM method in the temporalis muscles.Results:Gross and histological analyses showed that no significant pathological changes in the temporalis muscles in different experimental groups.Immunohistochemical staining demonstrated that c-jun was over-expressed in the nuclei of the muscles in the experimental groups,while negative or slight positive staining was observed in the nuclei of muscles in control animals.There was no obvious difference of c-jun expression in different experimental groups.Conclusion:Overload gravity can induce enhanced expression of c-jun in temporalis muscles of the monkeys received +Gx loads.
