Effects of arecoline on the expression of human telomerase reverse transcriptase in oral mucosa keratinocytes
- VernacularTitle:槟榔碱对口腔角质形成细胞端粒酶逆转录酶表达的影响
- Author:
Yijun GAO
;
Tianyou LING
;
Xiaomin YIN
- Publication Type:Journal Article
- Keywords:
Arecoline;
Keratinocytes;
Human telomerase reverse transcriptase
- From:
Journal of Practical Stomatology
2001;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of arecoline on the expression of human telomerase reverse transcriptase(hTERT)mRNA and protein in cultured normal human oral mucosa keratinocytes(KCs),and then to investigate the role of hTERT in carcinogenesis of oral submucous fibrosis(OSF).Methods:Normal human oral mucosa KCs were cultured in vitro,and in experiment group,KCs was divided into 0.03,0.06,0.09 g/L arecoline group,and 0.0 g/L arecoline group served as the control.The hTERT mRNA and protein expression of KC was examined by reverse transcription polymerase chain reaction(RT-PCR)and Western blotting.Results:Arecoline induced the hTERT mRNA and protein expression of KC in a dose dependent manner.hTERT mRNA and protein expression of KC were increased by 0.03,0.06 and 0.09 g/L arecoline,when compared with the control group(P
