Radiation Effect on NO, NOS and TGF-beta Expressions In Rat Lung.
- Author:
Young Taek OH
1
;
Kwang Joo PARK
;
Hoon Jong KIL
;
Mahn Joon HA
;
Mison CHUN
;
Seung Hee KANG
;
Seong Eun PARK
;
Sei Kyung CHANG
Author Information
1. Department of Radiation Oncology, School of Medicine, Ajou University, Suwon, Korea.
- Publication Type:Original Article
- Keywords:
Radiation pneumonitis;
NO;
NOS2;
TGF-beta;
Radiation
- MeSH:
Animals;
Lung*;
Radiation Effects*;
Radiation Pneumonitis;
Rats*;
Rats, Sprague-Dawley;
Therapeutic Irrigation;
Transforming Growth Factor beta*
- From:The Journal of the Korean Society for Therapeutic Radiology and Oncology
2000;18(4):321-328
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: NOS2 induce NO production and NO activate TGF-beta. The TGF-beta is a inhibitor of NOS2. If this negative feedback mechanism operating in radiation pneumonitis model, NOS2 inhibitor may play a role in TGF-beta suppression. We planned this study to evaluate the expression patterns of NO, NOS2 and TGF-beta in vivo radiation pneumonitis model. MATERIALS AND METHODS: Sixty sprague-Dawley rat were irradiated 5 Gy or 20 Gy. They were sacrificed 3, 7, 14, 28 and 56 days after irradiation. During sacrifice, we performed broncho-alveolar lavage (BAL). The BAL fluids were centrifuged and supernatents were used for measure NO and TGF-beta, and the cells were used for RT-PCR. RESULTS: After 5 Gy of radiation, NO in BAL fluid increased at 28 days in both lung and TGF-beta in left lung at 56 days. NO increased in BAL fluid at 28 days in both lung after irradiation and TGF-beta in right lung at 28-56 days after 20 Gy of radiation. After 5 Gy of radiation, NOS2 expression was increased in right lung at 14 days, in both lung at 28 days and in left lung at 56 days. TGF-beta expression was reduced in both lung at 28 days and increased in left lung at 56 days. CONCLUSIONS: The proposed feedback mechanism of NO, NOS2 and TGF-beta was operated in vivo radiation pneumonitis model. At 56 days, however, NOS2 and TGF-beta expressed concurrently in left lung after 5 Gy and in both lung after 20 Gy of radiation.
