Experimental study of the effect on the the distal femoral joint by the high intraosseous pressure induced with blocking of the proximal and middle femoral medullary cavity with bone cement
- VernacularTitle:骨水泥阻塞髓腔后骨内压升高对远侧关节影响的实验研究
- Author:
Hongyu LI
;
Hong AN
;
Bin LIANG
- Publication Type:Journal Article
- Keywords:
bone cement;
intraosseous hypertension;
distal joint;
degeneration or necrosis
- From:
Orthopedic Journal of China
2006;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
[Objective]To explore the effects on the distal femoral joint by high intraosseous pressure after blocking of proximal and middle femoral medullary cavity with bone cement.[Method]The rabbit model was established whose proximal and middle femoral medullary cavity of left side was infused with PMMA while the right side served as the control.Twenty-six rabbits were cavided randomly into 4 groups,8 for normal control group,the remained modeled rabbits were grouped according to the observing time-point into following groups:4th(T4),8th(T8) and 16th week(T16).The rabbits was killed to obtain the samples of distal femoral articular synovium,cartilage and the bone under cartilage.Then the structural changes of these tissues were observed by HE staining,toluidine blue staining,immunohistochemistry and transmission electron microscope.[Result](1)HE staining:the damage to articular cartilage,synovial tissue and the bone under cartilage aggravated with time.At the 16th week,it showed the articular cartilage was destructed,the bone structure was severely damaged with the synovial tissue proliferation and swelling.(2)Toluidine blue staining(16th week):all the layers of articular cartilage appeared loss of stain while the superficial layers were slightly loss of stain in control group.(3)Immunohistochemistry(16th week):①CollagenⅡ:the chondrocytes were positively stainned.②TGF-?1:chondrocytes stain was positive;the staining of articular synoviocytes appeared positive.(4)Transmission electron microscope:The lesions to articular chondrocytes and bone cells aggravated with time,part of which presented with necrosis and collapse at the 16th week.[Conclusion]The distal femoral intraosseous and intramedullary blood circulation is severely damaged after blocking of the proximal and middle femoral medullary cavity with bone cement,leading to continuous high intraosseous pressure.It makes the bone metabolism disordered in distal femur,which results degeneration and necrosis in distal femur,cartilage and synovial tissue.
