Experimental study on union culture of mesenchymal stem cell with fast setting calcium phosphate scaffold
- VernacularTitle:骨髓间充质细胞与速固化磷酸钙支架共培养的实验研究
- Author:
Jing WANG
;
Yuan ZHANG
;
Hua LIU
- Publication Type:Journal Article
- Keywords:
calcium phosphate;
chitosan;
bone marrow mesenchymal stem cells;
tissue engineering;
in vitro culture
- From:
Orthopedic Journal of China
2006;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
[Objective]To examine the effects of fast setting calcium phosphate scaffold on human mesenchymal stem cells(MSCs) adhesion and proliferation.[Method]Scaffolds were self-hardened in molds.Three ml human bone marrow aspirates,taken form the iliac crest of normal donors were suspended in low glucose DMEM supplemented with 10% fetal bovine serum.Atfer 72 hours the nonadherent cells were removed by changing medium,and the adherent layer cultured until it reached 80% conference.Cultured cells at passage 3 were used for the experiments.MSCs were seeded on calcium phosphate scaffold.The cell-scaffold constructs were cultured with complete media for 1 week.Scanning electron microscope(SEM)and the terazolium-based colorimetric assay(MTT test)examinations were performed.[Result]When cells were cultured in scaffolds in vitro,the number of cells on scaffold increased with time.SEM showed that the scaffold had a three-dimensional interconnected pore structure.The cells appeared to infiltrate into the macropores of the scaffold and the extracellular matrix components were observed.[Conclusion]This study demonstrates that the fast setting calcium phosphate scaffold has good biocompatibility,cells are able to adhere and proliferate on scaffold specimens,and it is a good substrate candidate for tissue engineered bone substitute.
