Establishment and characterization of a docetaxel-resistant variant of human lung adenocarcinoma cell line SPC-A1
- VernacularTitle:人肺腺癌细胞系SPC-A1多西他赛耐药细胞系的建立及其特性
- Author:
Hai SUN
;
Jian GENG
;
Jie JIN
;
Longbang CHEN
- Publication Type:Journal Article
- Keywords:
docetaxel;
lung adenocarcinoma;
drug resistance
- From:China Oncology
1998;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Background and qurpose:Docetaxel is one of the most effective chemotherapeutic agents developed in the past few years and has been used for the treatment of various cancers including lung cancer. Drug resistance to docetaxel is one of the main factors accounting for the failure of chemotherapy. This study was done to establish a human lung adenocarcinoma cell line SPC-A1 with the characterization of docetaxel resistance, to investigate its biological mechanism of drug resistance and how to reverse the resistance. Methods:A docetaxel resistant human lung adenocarcinoma cell line SPC-A1/Docetaxel was induced by continuously exposing human lung adenocarcinoma cell line SPC-A1 to gradually increasing doses of docetaxel. The multidrug resistance of SPC-A1/Docetaxel was evaluated by MTT assay. The distribution of cell cycle and rhodamine 123 accumulation in the two cell lines were detected by flow cytometry. Growth fraction was calculated by cytometry, and differentiation of genetics between drug resistance cell line and its parent cell line was analyzed by karyotype analysis.Results:Index number of drug resistance (IR) of SPC-A1/Docetaxel were 13.20, 2.18,1.12,1.39,1.38,0.93, 10.14 and 2.12 to docetaxel, paclitaxel, pharmorubicin, cisplatin,carboplatin, gemzar, vinorelbine and etoposide , respectively. Flow cytometry analysis showed G1/G0 arrest in SPC-A1/Docetaxel cell lines and there was no difference in terms of G2 cell proportion between SPC-A1/Docetaxel and SPC-A1 cell lines. Rhodamine concentration in SPC-A1/Docetaxel cells were markedly lower than that in SPC-A1 cells(806.34?0.49 Vs. 1382.26?0.32). Cell doubling time of SPC-A1/Docetaxel cells and SPC-A1 cells were 35.1 and 27.4 hours, respectively, with no difference in terms of genetics between the two cell lines.Conclusions:The SPC-A1/Docetaxel cell line showed high resistance to docetaxel, and had the characteristic of multidrug resistance. The cell line could be useful for the study of drug resistant mechanism and its reversal of docetaxel.
