Effects of Tripterygium Wlilfordii on renal interstitial fibrosis and inflamed lesion in UUO mice kidney
- VernacularTitle:雷公藤多苷对单侧输尿管结扎小鼠肾小管间质纤维化和炎性损伤的影响
- Author:
Ruchun YANG
;
Yongjun WANG
;
Dawei ZHOU
;
Jun WANG
;
Xiaoling ZHU
- Publication Type:Journal Article
- Keywords:
Tripterygium Wlilfordii;
?-smooth muscle actin;
Intercellular adhesion molecule 1;
Monocyte chemoattractant protein 1
- From:
Chinese Journal of Immunology
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of Tripterygium Wlilfordii on renal interstitial fibrosis and the expression of inflammatory factor in mice kidney with the Unilateral Ureteral Obstruction(UUO).Methods:Mice were randomly assigned to shame operation group(shame proup), UUO group, Tripterygium Wlilfordii and ACEI treatment group after UUO. The protein expression of ?-SMA,ICAM-1 were assessed by immunohistochemistry or Western blot; MCP-1 mRNA were detected by reverse transcription-polymerase chain reaction analyses(RT-PCR). And the change of renal pathology was observed at the same time.Results:In comparison with the shame group, the protein levels of ?-SMA, ICAM-1 and the mRNA expression of MCP-1 in UUO mice and treatment groups increased significantly, meanwhile Tripterygium Wlilfordii and ACEI could significantly reduce the expression of ?-SMA, ICAM-and MCP-1 on levels of protein and gene. The effects of Tripterygium Wlilfordii on ICAM-1 and MCP-1 were more significant than ACEI.Conclusion:Tripterygium Wlilfordii could reduce the transdifferentiation of renal interstitial cells and prevent renal interstitial inflammatory injury.
