Specific coalescent peptides binding with CD59 on HeLa cells screened by phage peptide library
- VernacularTitle:利用噬菌体肽库筛选HeLa细胞表面与人CD59结合的活性短肽
- Author:
Linlin CUI
;
Meihua GAO
;
Bing WANG
- Publication Type:Journal Article
- Keywords:
HeLa cell;
Peptide library;
Human CD59 protein;
Tumor escape
- From:
Chinese Journal of Immunology
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To search for carrier of short active peptide with binding to human CD59 of the surface of HeLa cell.Methods:Ph. D. 12 display peptide library was biopanning with HeLa cell high-expressing CD59. After competitive test, positive phage clones were identified by sandwich ELISA and sequenced.Results:10 phage clones were identified to have higher combination with CD59.Conclusion:The sequence of peptide with binding to CD59 has been obtained.That might lay a basis for design of short peptide of CD59 on tumor sneaking through.
