Culture and comparison of the morphology and function of dendritic cells from human peripheral blood monocytes with and without dexamethasone
- VernacularTitle:人外周血树突状细胞培养和地塞米松对其分化的影响作用
- Author:
Hongtao LI
;
Jin HUANG
- Publication Type:Journal Article
- Keywords:
Monocyte;
Dendritic cell;
Dexamethasone;
Cytokine
- From:
Chinese Journal of Immunology
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the morphology and function of the dendritic cells(DC) from human peripheral blood monocytes and the change in cell differentiation and maturation after DC treated with dexamethasone(Dex-DC).Methods:DCs were sorted from human monocytes by culturing in presence of cytokines(GM-CSF, IL-4) and LPS for 8 days with or without dexamethasone. The change in morphology, function and phenotypic characterization was compared.Results:The cells treated with or without Dex, developed a characteristic dendritic morphology, however, Dex-DC expressed higher level of CD14 and lower level of CD83 than the untreated cells. In addition, a low APC function was demonstrated by Dex-DC.Conclusion:DCs can be induced from peripheral blood monocytes in medium with cytokines; the cultured PS in presence of Dex are shown at a more immature stage, indicating that Dex modulates DC differentiation, maturation, and function.
