Study on the relationship between the expressions of glucocorticoid receptor ? and ? mRNA in peripheral blood mononuclear cell of patients with polymyositis/dermatomyositis and the glucocorticoid efficacy
- VernacularTitle:多发性肌炎/皮肌炎患者外周血单个核细胞中糖皮质激素受体?、? mRNA表达与糖皮质激素疗效相关性的研究
- Author:
Jifeng LIU
;
Aie XU
;
Fangji SONG
- Publication Type:Journal Article
- Keywords:
Polymyositis/dermatomyositis;
Glucocorticoid receptor;
Glucocorticoid;
Reverse transcription-polymerase chain reaction
- From:
Chinese Journal of Immunology
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the relationship between expressions of guococorticoid receptor(GR)? and ? mRNA in peripheral blood mononuclear cell(PBMC) of patients with polymyositis/dermatomyositis(PM/DM) and the glucocorticoid(GC) efficacy.Methods:Reverse transcription-polymerase chain reaction(RT-PCR) was used to semiquantite the expressions of GR? and ? mRNA in PBMC of 18 cases of PM/DM patients sensitive to GC,10 cases of PM/DM patients resistant to GC and 20 cases of health blood donors.The levels of cortisol in sera of PM/DM patients and of health donors were detected using radioimmunoassay.Results:There was no significant difference in the levels of seral cortisol among PM/DM patients sensitive to GC, resistant to GC and the health donors.The expressions of GR? mRNA in PBMC from PM/DM patients either sensitive to GC or resistant to GC were significantly lower those that of the normal control. Moreover,the expression of GR? mRNA in PBMC of PM/DM patients resistant to GC was significantly lower than that of patients sensitive to GC. While the expression of GR? mRNA in PBMC of PM/DM patients was significantly higher than that of the normal control,there was no significant difference of the expression of GR? mRNA between PM/DM patients sensitive and resistant to GC.Conclusion:The lower expression of GR? mRNA in PM/DM patients may be related to resistance to GC therapy,whereas there is no significant role of the expression level of GR? mRNA.
