Effect of Manganese on Apoptosis,p53 and Bcl-2 Expression in Spermatogenic Cells of Rats
- VernacularTitle:锰对大鼠生精细胞凋亡及p53和Bcl-2表达的影响
- Author:
Xianping ZHANG
;
Xiulian CAI
;
Qianxing WANG
- Publication Type:Journal Article
- Keywords:
Metals,heavry;
Manganese;
Sperm;
Apoptosis;
B-cell lymphoma/leukemia-2;
p53
- From:
Journal of Environment and Health
1992;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of manganese chloride on apoptosis,p53 and Bcl-2 expression in the spermatogenic cells of rats.Methods 24 healthy male SD rats were randomly divided into three groups.Two groups were treated with MnCl2?4H2O through intraperitoneal injection at 15 mg/kg and 30 mg/kg respectively,once a day and 5 times in a week for four weeks,the third group served as the control and given normal saline.The spermatogenic cell apoptosis was examined by transmission electronic microscope and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling(TUNEL)technique.Expression of p53 and Bcl-2 were measured with immunohistochemistry.Results The apoptosis index(AI)and p53-positive-cell rate in manganese exposed group were significantly higher than those in the control group(P
