Nickel Compound-induced DNA Damage in Human Lung Fibroblasts
- VernacularTitle:镍化合物对人胚肺成纤维细胞DNA的损伤
- Author:
Jing ZHANG
;
Jun ZHANG
;
Hongjun SHI
- Publication Type:Journal Article
- Keywords:
Environmental pollution;
Nickel;
Human lung fibroblasts;
DNA damage;
Comet assay
- From:
Journal of Environment and Health
1992;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the DNA damage in the human lung fibroblasts(HLF)induced by insoluble nickel compounds(Ni3S2 and Ni2O3)and soluble nickel compound(NiSO4),and to study the mechanism of carcinogenesis.Methods The DNA damage of HLF cells treated with different concentrations of Ni3S2,Ni2O3 and NiSO4 were detected with the method of single cell gel electrophoresis(SCGE).Results All of the three kinds of nickel compounds significantly caused DNA damage in HLF cells,the average tail moments of all nickel-treated groups were larger than those in the control group(P
