Vitamin K3 induces apoptosis of breast carcinoma cell line MCF-7
- VernacularTitle:维生素K3诱导乳腺癌细胞凋亡作用及调控因素的观察
- Author:
Peng SHI
- Publication Type:Journal Article
- Keywords:
Breast neoplasms?Vitamin K3?Catalase?Apopotosis
- From:
Chinese Journal of Current Advances in General Surgery
1999;0(02):-
- CountryChina
- Language:Chinese
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Abstract:
Objectives:To study the apoptosis-inducing effects of vitamin K3(VitK3) on human breast carcinoma cell line MCF-7,and approach its possible mechanism.Methods:MCF-7 is cultured in the presence of different concentration of VitK3 or in combination with catalase.The inhibitory effects of VitK3 and the influence of catalase were detected by MTT colorimetry.Apoptosis was observed using flow cytometry.The mRNA expression of RelA,Bcl-2,Bax and cyclin-dependent kinase inhibitor p21CIP1/WAF1,p27KIP1 were assessed by RT-PCR.Results:VitK3 can inhibit MCF-7 cell proliferation,and catalase significantly decreases the growth inhibition due to VitK3.Apoptosis is the reason of growth-inhibitory effect of VitK3.The mRNA expressions of RelA,Bax,p21CIP1/WAF1 were up-regulated after the treatment of VitK3.Conclusion:VitK3 can induce apoptosis of MCF-7 cell,at least in part by promoting the expression of Bax and inducing G1 arrest of cell cycle through up-regulation of p21CIP1/WAF1.
