Construction of tissue engineered skin by adipose tissue-derived stem cells and collagen for skin defect repair
- VernacularTitle:脂肪组织来源干细胞复合胶原构建组织工程皮肤修复皮肤缺损
- Author:
Wei LU
;
Yan JIN
;
Yongjie ZHANG
- Publication Type:Journal Article
- Keywords:
Adipose tissue-derived stem cell;
Tissue engineering;
Skin;
Wound healing
- From:
Journal of Practical Stomatology
1996;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the feasibility of adipose tissue-derived stem cells(ADSCs) as the seeding cells of skin engineering for skin defect repair. Methods :Adipose tissues were isolated from a SD rat groin, the fat was cut and incubated in a collagenase solution, and then the ADSCs were cultivated in culture medium. The amplified cells in vitro were inoculated in collagen gel to form tissue engineered skin. Fifteen SD rats were randomly divided into three groups: experimental group was treated with ADSCs collagen tissue engineered active skin substitute, and single collagen and blank were served as two control groups. The materials were grafted onto total skin defect wounds on the back of rats in the corresponding group respectively. The effect of wound repair was observed histologically. Results:The time of wound healing in the ADSCs and collagen experimental group, the single collagen control group and the blank control group were (14.3?1.7) days,(16.9?2.5)days,and (21.2?4.2)days respectively, and showed significant differences(P
