Genetic diversity in Fluconazole-resistant and -susceptible oral Candida albicans isolated from patients with Sjgren's syndrome
- VernacularTitle:舍格伦综合征患者口腔白色念珠菌耐药性与基因型的研究
- Author:
Zhimin YAN
;
Yanying XU
;
Hong HUA
- Publication Type:Journal Article
- Keywords:
Candida albicans;
Sjgren's syndrome;
Fluconazole;
Random amplification of polymorphic DNA
- From:
Journal of Practical Stomatology
1996;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyze genetic diversity of Fluconazole(FLZ)-resistant and -susceptible Candida albicans(C. albicans) strains isolated from patients with Sjgren's Syndrome (SS). Methods:30 C.albicans strains were isolated from the patients with SS and identified using standard criteria. Microdilution method was performed to determine the minimum inhibitory concentrations (MICs) of C.albicans to FLZ. A composite genotype was generated for each strain through random amplification of polymorphic DNA (RAPD) using three different primers, RSD10, RSD11 and RSD12. Results:The DNA fingerprinting profiles indicated genetic diversity amongst both the FLZ-resistant as well as -susceptible isolates, and no specific features emerged distinguishing the drug-resistant and -susceptible groups. Conclusion:These observations cast doubt on the theory of a clonal origin of FLZ-resistant C. albicans isolates. The emergence of FLZ resistance in SS patients may be associated with continuous exposure to FLZ.
