Effects of exogenous estrogen on the gene expression of genioglossus myosin heavy chain(MHC) in ovariectomized rats
- VernacularTitle:外源性雌激素局部应用对去势雌性大鼠颏舌肌肌球蛋白重链基因表达的影响
- Author:
Dan SHI
;
Yuehua LIU
;
Ying WANG
- Publication Type:Journal Article
- Keywords:
Genioglossus;
Estrogen;
Myosin heavy chain;
Ovariectomy
- From:
Journal of Practical Stomatology
1996;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the effect of exogenous estrogen on the gene expression of genioglossus myosin heavy chain(MHC) gene expression in ovariectomized rats. Methods: 30 female Sprague-Dawley rats were assigned to three groups randomly: ovariectomized group (OVX), estrogen group(E_2)and sham-operated group (SO). All the rats were sacrificed at 30 days after injection, and the genioglossus was removed for analysis. The gene expression of MHCⅡa,MHCⅡx, MHCⅡb and ?-actin was examined by real-time quantitative RT-PCR (FQ-PCR). Results: Compared to the SO group, genioglossus MHCⅡb increased significantly in OVX group (P
