Effect of Hepatocellular Carcinoma Cells Transfected with Inhibitory Kappa B Alpha on Expression of Nuclear Factor Kappa B and Matrix Metalloproteinase-9
- VernacularTitle:I?B-?基因转染HCC9204细胞对NF-?B和MMP-9表达的影响
- Author:
Kejun ZHANG
;
Yanming GAO
;
Jinyong YANG
;
Bingyuan ZHANG
- Publication Type:Journal Article
- Keywords:
HCC9204 cell Cell transfected Inhibitory kappa B alpha Nuclear factor kappa B Matrix metalloproteinase-9
- From:
Chinese Journal of Bases and Clinics in General Surgery
2003;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression changes of nuclear factor kappa B (NF-?B) and matrix metalloproteinase-9 (MMP-9) in the cultured hepatocellular carcinoma cells 9204 (HCC9204) transfected with inhibitory kappa B alpha(I?B-?)vector. Methods After pcDNA3-I?B-? vector and pcDNA3 were transfected into HCC9204 by lipofectamine method, Western-blot and RT-PCR analysis were used to detect the expressions of NF-?B and MMP-9. Migration and invasion of tumor cells were assayed by fundus membrane invaded by them. Results When pcDNA3-I?B-? was transfected into HCC9204, the expression of NF-?B was decreased at the protein level, and the expression of MMP-9 mRNA and the invision and metastasis ability of transfected cells were obviously decreased. Conclusion When the activity of NF-?B is inhibited, the ability of invasion and metastasis in HCC9204 cells decrease, which could be related to the decreased the expression of MMP-9.
