Oxidant-induced DNA damage and the reparation in NIH3T3 mouse fibroblasts by single cell gel electrophoresis
- VernacularTitle:单细胞凝胶电泳技术检测小鼠成纤维细胞DNA的氧化损伤及修复
- Author:
Xin CHEN
;
Nishida HIROSHI
;
Konishi TETSUYA
- Publication Type:Journal Article
- Keywords:
single cell gel electrophoresis technique(SCGE);
single strand breaks(SSB);
oxidant-induced DNA damage;
DNA reparation
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
1982;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study H2O2-induced single strand breaks(SSB) formation in DNA and the reparation in NIH3T3 mouse fibroblasts by single cell gel electrophoresis technique(SCGE,Comet Assay) and to introduce SCGE.Methods The NIH3T3 mouse fibroblasts were incubated,and cell damage was induced by H2O2 and determined by SCGE.Results ① H2O2-induced damage class graph of the DNA Comet in the NIH3T3 mouse fibroblasts was established.② H2O2-induced SSB was H2O2 dose-dependent.③ The reparation of the damaged DNA was time-dependent and could be achieved 15min after incubation.The data indicated that most reparations were completed within 1 hour,but a few might take longer time.Conclusion Single cell gel electrophoresis technique is a simple and sensitive method to determine oxidation-induced single strand breaks(SSB) formation in DNA.
