Culture,proliferation and differentiation of human marrow mesenchymal stem cells to cardiomyocytes in vitro
- VernacularTitle:人骨髓间充质干细胞体外培养扩增及向心肌细胞诱导分化的实验研究
- Author:
Fangge DENG
;
Xiuying ZHANG
;
Limei QU
;
Yulin LI
- Publication Type:Journal Article
- Keywords:
Bone marrow;
Human mesenchymal stem cells(hMSCs);
5-azacytidine;
Cardiomyocytes
- From:
Chinese Journal of Immunology
1985;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
95% purity were treated for 24 h with 1,5,10 and 20 ?mol/L 5-azacytidine to induce cellular differentiation.Expression of the cardiac-specific marker-troponin Ⅰ and desmin,identified by immunohistochemistry,was used to identify cardiac muscle differentiation.Results:hMSCs expressed a high level of CD44(hMSCs 93.26%?2.48% vs 3.42%?1.09% in a control group,P
