Expression and purification of murine ?-1,3-galactosyltransferase catalytic domain
- VernacularTitle:鼠源性?-1,3-半乳糖基转移酶催化结构域的表达及纯化
- Author:
Rui ZHANG
;
Baorong CHI
;
Xuewei ZHU
;
Junyi WANG
- Publication Type:Journal Article
- Keywords:
?-1,3-galactosyltransferase;
?-gal epitopes;
Catalytic domain;
pET-15b
- From:
Chinese Journal of Immunology
1985;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To express and purificate catalytic domain of murine?-1,3-galactosyltransferase and provide the feasible method in the tumor cell surface production ?-gal epitopes..Methods:This research established expression system in pET-15b to express catalytic domain of murine ?-1,3-galactosyltransferase,then identified its activity by HPLC with anion exchange column.Results:(1)Constructed successfully recombinant ?-1,3-galactosyltransferase catalytic domain with His-tag.(2)?-1,3-galactosyltransferase with His-tag in a soluble form was expressed and purificated effeciently.(3)Its activity by HPLC with anion exchange column showed.Conclusion:This research shows ?-1,3-galactosyltransferase in a soluble form has been expressed successfully.
