Effects of mesenchymal stem cells intravenous transplantation on the glial cell line derived neurotrophic factor after the spinal cord injury of rats
- VernacularTitle:静脉移植骨髓间充质干细胞促进脊髓损伤后GDNF基因的表达
- Author:
Guoliang LI
;
Jun CHEN
;
Songwei YANG
- Publication Type:Journal Article
- Keywords:
spinal cord injury;
mesenchymal stem cells;
glial cell line derived neurotrophic factor
- From:
Orthopedic Journal of China
2006;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of mesenchymal stem cells (MSCs)injection from tail-vein on the expression of glial cell line derived neurotrophic factor (GDNF) after the spinal cord injury (SCI) of rats.MethodMSCs were cultured from the thighbone of adult wistar rats.The T10 level spinal cord injury was execute by the modify-Allen's device. The MSCs labeled by bromodeoxyuridine were transplanted into the vein of tail by injection immediately after spinal cord injury. Adult Wistar rats were randomly divided into three groups: SCI cured with MSCs transplantation (group A), SCI received normal saline(group B),and control group (group C). Then MSCs were detected and the expressions of GDNF of the lesion and neighbor areas were examined by Reversetranscription polymerase chain reaction (RT-PCR) and immunohistochemistry.ResultImmunohistochemistry: MSCs labeled by bromodeoxyuridine could be detected in the injuried spinal cord after transplantation. It was located at the nucleolus of MSCs. The cells survived and migrated rostrallyand caudally from the injection sites. With the time passed, the number of MSCs labeled with bromodeoxyuridine was decreased. RT-PCR: The expression of Group A was increased on the followed day, and the expression of Group B was increased on the 1st day but decrease from the 5th day then on.compared with group B, transplantation of MSCs significantly enhanced the expression of GDNF mRNA than group A on the 1st, 3rd, 5th day after cell transplantation(P
