Oral bacterial biofilm comparative susceptibility of various extraction compounds from Galla Chinensis
- VernacularTitle:五倍子不同组分对口腔细菌生物膜的清除效应
- Author:
Jin ZHAO
;
Bing ZHU
;
Xuedong ZHOU
- Publication Type:Journal Article
- Keywords:
Galla Chinesis;
Mouth;
Bacteria;
Biofilm;
Minimal biofilm eradication concentration
- From:
Journal of Practical Stomatology
2001;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To assay minimal biofilm eradication concentration (MBEC) of various extraction compounds from Galla Chinensis to 5 oral bacterial biofilms, and then to set up traditional Chinese medicine empirical study methods of pharmacodynamic susceptibility. Methods:5 cariogenic bacteria strains were selected (Streptococcus mutans ATCC25175, Streptococcus sanguis ATCC10556, Lactobacillus rhamnosus AC413, Actinomyces naeslundii WVU627, Actinomyces viscosus ATCC19246) in present study. Various extraction compounds of Galla Chinensis were GCE (aqueous extract), GCE-B(300 ml/L alcohol extract), GCE-C(500 ml/L acetone extract),GCE-D(1 000 ml/L acetone extract), gallic acid and methyl gallate. ①Observed oral bacterial biofilm formation in MBECTM-Device at different-time-stages. ②MBECTM -HTP-Assay:The minimal inhibitory concentration (MIC) and minimal biofilm eradication concentration (MBEC) of various extraction compounds of Galla Chinensis on 5 oral bacteria strains were determined. Results:5 oral bacterial biofilm were readily formed on the lid of MBECTM-Device under selected condition (observed by SEM). 5 oral cariogenic bacteria growing as planktonic populations were sensitive to 6 extraction compounds of Galla Chinensis. When compared to 6 extraction compounds of Galla Chinensis, 5 oral cariogenic bacterial biofilm were 2-16 times less susceptible than growing planktonic bacteria. GCE and GCE-B are the most effective medicine against 5 oral cariogenic bacterial biofilm. GCE-C and GCE-D were effective in killing oral-bacterial biofilm at relatively high concentration, but gallic acid and methyl gallate were less effective.Conclusion:GCE and GCE-B are the most effective medicine against 5 oral cariogenic bacterial biofilm of 6 extraction compounds from Galla Chinensis. MBEC can provide a relatively accurate medicine concentration for clinical test.
