Significance of Expression of Platelet Derived Growth Factor and Survivin of Hepatocellular Carcinoma with Portal Vein Tumor Thrombosis
- VernacularTitle:肝细胞癌合并门静脉癌栓组织中PDGF和survivin表达的意义
- Author:
Zhefu LI
;
Xiaoping CHEN
;
Wanguang ZHANG
- Publication Type:Journal Article
- Keywords:
Hepatocellular carcinoma Platelet derived growth factor Survivin gene Portal vein Tumor thrombus
- From:
Chinese Journal of Bases and Clinics in General Surgery
2004;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expressions of platelet derived growth factor (PDGF) and survivin in hepatocellular carcinoma (HCC) with portal vein tumor thrombosis (PVTT) and to evaluate the relationships among the expressions of PDGF, survivin and the proliferation of cancer cells. Methods The expression of PDGF mRNA in 16 cases in HCC with PVTT group was observed by in situ hybridization and the results were compared with that in HCC tissue group. The expressions of PDGF and survivin protein in 36 cases in HCC with PVTT group were detected with immunohistochemistry and it was found that there was a correlation between them. Flow cytometry (FCM) was used to measure the proliferation of cancer cells and it was also used to analyze the relations among PDGF, survivin and the proliferation of cancer cells. Results The expression level of PDGF mRNA in HCC with PVTT group was significantly higher than that in HCC tissue group (P
