Role of Apoptosis in Acute Rejection of Pancreaticoduodenal Transplantation in Rats
- VernacularTitle:细胞凋亡在大鼠胰腺移植急性排斥反应中的作用
- Author:
Tao LI
;
Jun LUO
;
Hao WEN
- Publication Type:Journal Article
- Keywords:
Pancreas transplantation Rejection Apoptotic gene Duodenum biopsy
- From:
Chinese Journal of Bases and Clinics in General Surgery
2004;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the roles of cell apoptosis and the gene expressions of Fas, FasL, bcl-2 and bax in acute rejection of pancreaticoduodenal transplantation and to evaluate the function of duodenum biopsy for early detection of rejection in rats. Methods Wistar and SD rats were divided into two groups: ①Wistar rats that underwent allogenic pancreaticoduodenal transplantation from the organs of SD rats; ②Wistar rats that received homogenic transplantation. The grafts were then harvested on day 3, 5 and 7 after the transplantation, and all graft samples were observed with HE staining and TUNEL was also used to detect apoptotic cells. The expressions of Fas, FasL, bcl-2 and bax were measured by immunochemical method. According to Nakhleh’s score, pathologic features of transplanted pancreas and duodenum were ranged from one to three scores in order. Results The percentage of same or different scores between the pathological scores of pancreas and duodenum in allogenic pancreaticoduodenal transplantation group were 61.1% (11/18) and 38.9% (7/18) respectively, and there were 6 specimens of pancreatic tissue got higher scores with only one higher score for duodenum. There were significant differences of histopathologic rejection scores and apoptotic indices between the two groups, respectively (P
