Effects of Survivin Antisense RNA on Apoptosis and Reversing Drug Resistance of SGC7901 Cells
- VernacularTitle:反义survivin诱导胃癌SGC7901细胞凋亡及逆转耐药的研究
- Author:
Xihai CHEN
;
Qifan ZHANG
;
Rong MA
- Publication Type:Journal Article
- Keywords:
Survivin Antisense Apoptosis Taxotere Multi-drug resistance gene
- From:
Chinese Journal of Bases and Clinics in General Surgery
2004;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of survivin antisense RNA on SGC7901 cell’s apoptosis and chemosensitivity to taxotere, and to investigate its effect on the expression of multi-drug resistance gene-1 (MDR-1). Methods Survivin antisense eukaryotic vector anti-pcDNA3-svv was transfected into SGC7901 cell lines by lipofectamine and positive clones were screened out then. Survivin protein and MDR-1 mRNA were measured by western blot and RT-PCR, respectively. Apoptosis that was induced by anti-pcDNA3-svv was observed by electronic microscope, and the sensitivity of SGC7901 cell to taxotere was examined by MTT. Results The expressions of survivin protein and MDR-1 mRNA in transfected SGC7901 cells both decreased more significantly than that of non-transfected cells (P
