ras Gene Damage Induced by Organic Concentrate of Drinking Water
- VernacularTitle:氯化消毒饮用水浓集物对ras基因的DNA损伤作用
- Author:
Chaolong RAO
;
Xufang GAO
;
Zhengchang HENG
- Publication Type:Journal Article
- Keywords:
DNA damage;
Genes, ras;
Organic concentrate of drinking water;
Randomized terminal linker-dependent PCR
- From:
Journal of Environment and Health
1993;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect the DNA damage of ras gene induced by organic concentrate of drinking water using randomized terminal linker-dependent PCR. Methods The single-stranded probes of exons of rass gene in human were prepared. The TK6 cell was treated with organic concentrate of drinking water, then genomic DNA was amplified by RDPCR and detected by Southern hybridization with the probe. Results The clear hybridized band induced by organic concentrate of tap water could be seen in the position of exon 2 of N-ras gene,but in those of exon 1 of N-ras, exon 1 and 2 of H-ras gene it could not be detected. No hybridized bands induced by organic concentrate of raw water were seen in any position. Conclusion The organic concentrate of drinking water can cause the DNA damage of ras gene and it is considered as a kind of genotoxic substance.
