Synthesis and identification of the conjugation of nonylphenol and ovalbumin
- VernacularTitle:壬基酚与卵清蛋白的结合与鉴定
- Author:
Tao WANG
;
Zhixian GAO
- Publication Type:Journal Article
- Keywords:
Nonylphenol;
Ovalbumin;
Formaldehyde
- From:
Chinese Journal of Immunology
1985;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To conjugate nonylphenol and ovalbumin(OVA).Methods:Nonylphenol was conjugated to OVA by Mannich reaction with formaldehyde in phosphate buffer(PBS, pH=8.0).And we identified the conjugate by antichip and ultraviolet scan.Results:Nonylphenol was conjugated to OVA successfully, and nonylphenol monoantibody recognized the conjugate less than 2.68 ?g/ml.Conclusion:This is an easy method to produce the conjugate of nonylphenol successfully.