Significance of quantitative detecting perforin mRNA expression in the diagnosis of rat acute rejection
- VernacularTitle:定量检测穿孔素表达在大鼠移植肾急性排斥反应诊断中的意义
- Author:
Lixin YU
;
Ruotong HUANG
;
Xiaoyou LIU
;
Shaojie FU
;
Xuyong SUN
;
Qian YUAN
- Publication Type:Journal Article
- Keywords:
Fluorescent quantitative PCR;
Acute rejection;
Perforin;
Rats
- From:
Chinese Journal of Urology
2006;0(S1):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To quantitatively detect intragraft and peripheral blood monocyte cells (PBMCs) perforin mRNA,and analysis the relationship between acute rejection and perforin mRNA. Methods In the allograft group Wistar and male SD rats were used as donors and recipients.SD rats served as donors and recipients in the isograft group.The graft specimens were harvested 5 days postoperatively.They were examined histologically after sectioning and staining with hematoxylin and eosin,Masson’s trichrome, periodic acid-Schiff reagent and periodic acidsilver metheramine.And then the fluorescent quantitative PCR was employed to measure the intragraft and PBMCs expression level of perforin mRNA. Results The histological scores in allograft and isograft kidney samples were 2~5 (3.43?0.98) and 0~1 (0.71?0.49)(P
