Effect of vitamin E on detrusor protection in BOO rabbits
- VernacularTitle:维生素E对膀胱出口部分梗阻后膀胱功能的保护作用
- Author:
Bin JIN
;
Xiaofeng WANG
;
Yaming CHU
;
Zheng YAN
;
Xiaodong ZHANG
- Publication Type:Journal Article
- Keywords:
Bladder outlet obstruction;
Bladder function;
Vitamin E;
Sarcoplasmic endoplasmic reticulum,calcium,magnesium,adenosine triphosphatase (SERCA2)
- From:
Chinese Journal of Urology
2006;0(S1):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the protective effect of vitamin E on the rabbit bladder after partial outlet obstruction artificially setup. Methods A total of 28 New Zealand white male rabbits were divided into 4 groups (group A in 6,group B in 6,group C in 8 and group D in 8).Group A,B and C were fed a regular diet,and group D were placed on a diet enriched with 600 mg vitamin E.After 4 weeks partial outlet obstruction was created in groups C and D,while group B underwent sham operation. After 4 weeks of obstruction each rabbit was sedated and cystometry was repeated.After cystometry the bladder was weighed.The gene expression of sarcoplasmic endoplasmic reticulum,calcium,magnesium,adenosine triphosphatase (SERCA2) in bladder was detected by using RT-PCR assay,while the protein level of SERCA2 was measured by Western blot analysis. Results All parameters measured were approximately identical in nomal rabbits(Group A) and shum operation rabbits(group B).Thus,these 2 groups were combined as the control group(Group A and B).Partial outlet obstruction resulted in bladder weight increased significantly in obstructed groups given vehicle group C(13.07?1.71)g and those vitamin E group D(11.80?2.01)g,4-fold higher than in the control group (2.81?0.30)g(P
