Effects of glucose,insulin and uric acid on the expression of hemoglobin oxygenase in pig renal tubule cell line
- VernacularTitle:葡萄糖、胰岛素和尿酸对猪近端肾小管上皮细胞株血红素氧合酶-1的影响
- Author:
Hui SUN
;
Xiaomiao LI
;
Baili SONG
;
Xinping LIU
;
Libo YAO
;
Qiuhe JI
- Publication Type:Journal Article
- Keywords:
uric acid;
glucose;
insulin;
heme oxygenase;
pig near renal tubular cells line(LLC-PK1)
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
2003;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of metabolically correlated factors on pig renal tubule in cellular level by observing effects of glucose,insulin and uric acid on activity of haemachrome oxygenase in renal tubular epithelial cells.Methods Pig near renal tubular cell line(LLC-PK1) was selected as experimental object.After stimulating cells respectively with glucose(5,10,22,33mmol/L),uric acid(0,0.1,0.2,0.4mmol/L);and insulin(0,10~(-9),0~(-8),10~(-7)mol/L) in different concentrations for 48 hours,the activity of HO-1 within the cells was determined.Results In uric acid group the activity of HO-1 was significantly increased in a concentration-dependent manner,while in glucose and insulin groups,the activity of HO-1 did not change.Conclusion After LLC-PK1 was stimulated by uric acid of different concentrations,HO-1 was induced evidently,which indicates that uric acid might affect oxidative stress in renal tubule cell line.
