Effect of Bcl-2 and Bax protein expression on the apoptosis of renal tubular epithelia in rats with obstructive jaundice
- VernacularTitle:Bcl-2和Bax对梗阻性黄疸大鼠肾小管上皮细胞凋亡的调节作用
- Author:
Yunfeng ZHANG
;
Zuoren WANG
;
Shuiping HAN
;
Liming ZHENG
- Publication Type:Journal Article
- Keywords:
obstructive jaundice;
apoptosis;
Bcl-2 protein;
Bax protein
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
2003;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the relationship of the expression of Bcl-2 and Bax protein with apoptosis of renal tubular epithelia in rats with obstructive jaundice(OJ).Methods A total of 60 adult Sprague-Dawley(SD) rats were divided into two groups: OJ rats by ligating bile duct(OJ group,n= 40) and the sham-operation rats(the control group,n=20).The two groups of rats were sacrificed at postoperative 1,2,3 and 4 week,respectively.Then serum BUN and Cr levels were tested and renal histopathological and ultrastructural changes were observed.Apoptosis of renal tissues was assayed by TUNEL method.The immunohistochemical Elivision~(TM) technique was adopted for detecting the Bcl-2 and Bax protein expression.Results With the time of OJ prolonging,the value of serum BUN and Cr increased;apoptotic cells of renal tubular epithelia increased.There were obvious differences in Bcl-2 and Bax protein expression of between OJ and control groups(P
