The effect of bradykinin on the signaling of PGE_2 in cultured bovine trabecular meshwork cells in vitro
- VernacularTitle:缓激肽对体外培养的牛眼小梁细胞前列腺素E_2信号转导的影响
- Author:
Ying XIAO
;
Dexiu ZHANG
;
Huiqin LU
- Publication Type:Journal Article
- Keywords:
bovine trabecular meshwork cell;
bradykinin;
prostaglandinE_2;
cAMP;
glaucoma
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
2003;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To understand the influence of bradykinin(Bk) on the signaling of prostaglandin E_2(PGE_2) in cultured bovine trabecular meshwork cells(TMCs),and to discuss the mode of interaction between the two signal pathways in TMCs.Methods TMCs were incubated in 1000nmol/L PGE_2 or 1000nmol/L PGE_2 combined with 100nmol/L Bk for 60s.The production of intracellular cAMP was assayed by radioimmunoassay.Results Bk could magnify the stimulation of PGE_2 on cultured TMCs.PGE_2 combined with Bk could cause greater elevation of intracellular cAMP than PGE_2 alone(P
