Study of the gene loss during the developing of cervical squamous cell carcinoma
- VernacularTitle:子宫颈鳞状细胞癌发生过程中基因丢失的研究
- Author:
Xingyan LI
;
Quanhong WANG
;
Yang DENG
- Publication Type:Journal Article
- Keywords:
Loss of genes;
Cercival squamous cell carcinoma
- From:
Cancer Research and Clinic
2006;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the regulation of gene variation and explore the tumor associated suppressor genes on chromosome 9 during the developing of cervical squamous cell carcinoma. Methods Microdissection, PCR, electrophoresis and radiograph were selected to detect the LOH in 46 cases cervical squamous cell carcinoma with high-grade squamous dysplasia and normal tissue. The changes of LOH at chromosome 9 total seven microsatellite markers and relationship between LOH rate and clinical parameters were analysed. Results Total frequency of LOH in CIN (Ⅱ,Ⅲ) was 16 % and in cervical squamous cell carcinoma was 25 %. In the former, LOH at marker D9S171(30 %), D9S162(23 %), D9S43(20 %), D9S303(17 %), D9S753(12 %), D9S242(11 %) were found, whereas D9S1748 LOH was not detected in high-grade dysplasia. In the latter, LOH at marker D9S171(41 %), D9S43(33 %), D9S162(31 %), D9S242(24 %), D9S303(17 %), D9S753(17 %), D9S1748 13 % were found. In addition, LOH was found in high-grade dysplasia in three cases but not in cervical squamous cell carcinoma. Conclusions The study in seven microsatellite markers showed that from normal squamous tissue to dysplasia to cervical squamous cell carcinoma were accompanied with accumulation of gene errors. The p15 gene inactivation had a high relationship with the occur of cervical squamous cell carcinoma. Tumor suppressor genes associated with cervical squamous cell carcinoma may exist near or at D9S43, D9S162, D9S242. Some cases showed that high-grade dysplasia and cervical squamous cell carcinoma may came from different independent clone which provide some clue for multiple foci carcinoma at genetic level.
