Study of the differentiation of BMSCs induced by the OECs conditioned medium
- VernacularTitle:嗅鞘细胞条件培养液对骨髓基质干细胞的诱导分化作用研究
- Author:
Linjie FENG
;
Zhuojing LUO
;
Xiaoren WANG
- Publication Type:Journal Article
- Keywords:
bone marrow stromal cells;
olfactory ensheathing cells;
co-culture;
differentiation
- From:
Orthopedic Journal of China
2006;0(24):-
- CountryChina
- Language:Chinese
-
Abstract:
[Objective]To explore the differentiation of bone marrow stromal cells(BMSCs) induced by OECs conditioned medium.[Method]The purified OECs were acquired by seleclive culture medium and cultivated in the polylysine coated plate.After 9～12 days culture, OECs conditioned medium was obtained by 2000 r/min configuration and then co-cultivated with purified BMSCs which were 3th generation.The differentiation of BMSCs were observed under inverted microscope and were identified by immumofluorescence method.[Result]The OECs conditioned medium had obviously effects on BMSCs' differentiation.BMSCs differentiation into neuron-like cells ratio was 55% and differentiation into astroctes cells ratio was 23%.[Conclusion]The conditioned medium which comes from OECs culture supernatant has obviously effects on BMSCs' differentiation into neuron-like cells.
