Experimental study of rabbit segmental radial defects repaired by mesenchymal stem cells composite grafts
- VernacularTitle:骨髓间充质干细胞及其复合物修复兔桡骨缺损的实验研究
- Author:
Ming LI
;
Yujiang CAO
;
Dewen ZHANG
- Publication Type:Journal Article
- Keywords:
bone marrow mesenchymal stem cells(MSCs);
antigen-extracted bovine cancellous bone(BCB);
cell culture;
bone transplantation;
bone defect
- From:
Orthopedic Journal of China
2006;0(24):-
- CountryChina
- Language:Chinese
-
Abstract:
[Objective]To discuss the tissue engineering method of treating bone defects through rabbit bone marrow mesenchymal stem cells(MSCs)'culture in vitro and make composite grafts with anti-extracted bovine cancellous bone(BCB).The composite grafts were implanted into segmental radial defects in rabbits was compared on the curative effect of repairing segmental bone defects in rabbits with the composite grafts.[Method]The purified,culture-expanded MSCs were combined with BCB in vitro according to condition of cell culture.The composite grafts were tested by scanning electron microscope.An bone defect(15mm in length) was created at two radii in each rabbit.The composite grafts were implanted into segmental radial defects in rabbits through open operation.The curative effect was evaluated by radiographic examination,histologic analysis,biomechanical test after surgery in experimental groups and control's and blank's.[Result]Roentgenographically,the bone defects that had been treated with grafts exhibited new bone formation increased with time.But the result of experimental groups were apparently superior to that of the control groups′s at 2、4、8、12、16 week after operation(P
