The effect of anti-CD4-epirubicin to T lymphoma in vitro and in vivo
- VernacularTitle:抗CD4单抗-表阿霉素在体外和体内对T源性淋巴瘤的作用
- Author:
Ling ZHANG
;
Tao DONG
;
Libing SONG
- Publication Type:Journal Article
- Keywords:
Anti-CD4 monoclonal antibody;
Epirubicin;
CEM cell;
Immunoconjugate;
~(125)I-anti-CD4 conjugate
- From:
Chinese Journal of Immunology
1999;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To detect the specificity killing effect of anti-CD4-epirubicin immunoconjugate on T origin lymphoma cells.Methods:The immunoconjugate was made by linking anti-CD4 monoclonal antibody with epirubicin using Dextran T10 as linkage. The affinity and the killing effect of the immunoconjugate in vitro and in vivo were detected by complement independent cytotoxicity, immuno-histochemistry, CFU-GM and ~ 125 I-anti-CD4 radio-immuno-imaging in nude mice.Results:Anti-CD4 immunoconjugate showed a strong affinity and specific killing effect to T origin lymphoma CEM cells.Conclusion:Anti-CD4-epirubicin and ~ 125 I-anti-CD4 could be used for diagnosis and treatment of T origin lymphoma.
