Preparation of monoclonal antibody against human lung cancer and purification of its antigen by the immunoaffinity chromatography
- VernacularTitle:人肺癌相关单克隆抗体的制备及其抗原的纯化
- Author:
Xiaoling YAO
;
Xiaoyan LIU
;
Qiang WU
;
Zhenshan XU
;
Lihua SONG
- Publication Type:Journal Article
- Keywords:
Monoclonal antibody;
Lung cancer;
Immunoaffinity chromatography
- From:
Chinese Journal of Immunology
1999;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To produce the mcAb specifically reacting with lung cancer and to purificate its antigen.Methods:The mice were immunized with A549, the mcAb 2B9 was screened by indirect cell ELISA and immunohistochemistry, and its antigen was purificated by immunoaffinity chromatography.Results:A mcAb was obtained, which could react to lung cancer but very little or not to normal lung tissue and other caner tissues, and the antigen of the mcAb was purificated from the cell lysate.Conclusion:A mcAb which can react to lung cancer have been obtained and its antigen was purificated, they may be useful on clinic for diagnosis and prognosis of lung cancer.
