Investigation of psoriatic T lymphocytes action on Ki67,c-Myc and Bcl-xL protein expression of keratinocytes in vitro
- VernacularTitle:体外研究银屑病患者外周血T淋巴细胞对角质形成细胞Ki67、c-Myc及Bcl-xL蛋白表达的影响
- Author:
Guohua YIN
;
Xing FAN
;
Kaiming ZHANG
;
Xinhua LI
- Publication Type:Journal Article
- Keywords:
Psoriasis;
T lymphocytes;
Proteins Ki67;
Proto-oncogene proteins c-Myc;
Proteins Bcl-xL
- From:
Chinese Journal of Immunology
1999;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To reveal the action of psoriatic peripheral blood T lymphocytes on keratinocytes proliferation and the significance in the pathogenesis of psoriasis.Methods:Keratinocytes were cocultivated with psoriatic peripheral blood T lymphocytes in comparison with those cocultivated with normal T lymphocytes. Immunohistochemical technique was performed to detect the expression of Ki67, c-Myc and Bcl-xL proteins.Results:There were significant overexpression of Ki67, c-Myc and Bcl-xL proteins in keratinocytes cocultivated with psoriatic T lymphocytes compared with the uncocultivated group and normal T lymphocytes group. Expression of Ki67, c-Myc and Bcl-xL proteins in keratinocytes cocultivated with normal T lymphocytes were not significantly different from the uncocultivated group.Conclusion:Psoriatic T lymphocytes, which have specific activity, can induce keratinocytes abnormal pattern of proliferation. One of the important mechanisms might be its action on the expression of c-Myc and Bcl-xL proteins.
