Regulatory Effects of Fenofibrate with Inflammatory Response and Myocardiac Dysfunction in Lipopolysaccharide-stimulated Heart Tissues.
- Author:
Dong Hoon SONG
1
;
Yong HWANG
;
Su Jin YOO
Author Information
1. Department of Emergency Medicine, Wonkwang University College of Medicine, Iksan, Korea. ysoojin@wmc.wonkwang.ac.kr
- Publication Type:Original Article
- Keywords:
Sepsis;
Lipopolysaccharide;
Cardiac dysfunction;
Fenofibrate
- MeSH:
Animals;
Blotting, Western;
Catalase;
Cytokines;
Fenofibrate;
Heart;
Immunohistochemistry;
Interleukin-1beta;
Interleukin-6;
Lipid Peroxidation;
Mice;
Peroxisome Proliferator-Activated Receptors;
PPAR gamma;
Sepsis;
Transcription Factors;
Tumor Necrosis Factor-alpha
- From:Journal of the Korean Society of Emergency Medicine
2012;23(5):721-729
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: This study was intended to establish experimental conditions for monitoring the cardioprotective effects of fenofibrate on cardiac function in lipopolysaccharide (LPS)-stimulated BalB/c mice. METHODS: To investigate the effects of fenofibrate on cardiac function, expression of Peroxisome proliferator-activated receptors (PPARs) and Peroxisome proliferator-activated receptor Gamma coactivator 1(PGC-1) and its target gene in the heart tissues of mice was compared after controls and LPS injection with pretreated fenofibrate or alone using reverse transcriptase-polymerase chain reaction (RT-PCR), Western blot analysis, and immunohistochemistry. In addition, Enzyme-linked-immunosorbent-assays (ELISA) were performed for assessment of pro-inflammatory cytokines of blood serum. RESULTS: Pretreated with fenofibrate had protective effects of diminishing the levels of LPS-induced pro-inflammatory cytokines, including interleukin-1beta (IL-1beta), interleukin-6 (IL-6), and tumor necrosis factor-alpha (TNF-alpha) and recovery from reduction of messenger Ribo-nucleic acid, protein level of PPARs and PGC-1 in LPS-administered heart tissue. In addition, increasing expression of PPARs and PGC-1 ameliorated the expression and activity of catalase blocked production of lipid peroxidation. CONCLUSION: Treatment with fenofibrate resulted in augmented expression of transcription factors and reduced production of pro-inflammatory cytokines and lipid peroxidation after LPS administration. Therefore, results of this study suggested that fenofibrate should not only have a protective effect but should also restore cardiac function in several cardiac dysfunctional situations.
