Preparation of Bone Marrow Dendritic Cells with TNF-? and The Immune Response Against Malignant Pancreatic Cell by Dendritic Cell Vaccine
- VernacularTitle:TNF-?增强树突状细胞诱导的抗胰腺癌免疫应答的实验研究
- Author:
Guang TAN
;
Jinshuai REN
;
Zhongyu WANG
;
Shuo YIN
- Publication Type:Journal Article
- Keywords:
Dendritic cell Pancreatic carcinoma Immune response
- From:
Chinese Journal of Bases and Clinics in General Surgery
2003;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the method of obtaining a large number of dendritic cells (DC). To study the specific cytotoxicity T lymphocyte (CTL) effect against tumor cells initiated by DC pulsed with peptide of cancer cell. Methods Development of cells with cytologic features of DC in bone marrow cultures supplemented with granulocyte macrophage-colony stimulus factor (GM-CSF) and IL-4. Determining the DC phenotype and the specific structure by electronic microscopy. The CTL effect against pancreatic carcinoma leading by the DC pulsed with tumor cells lysate in vitro was observed. Results A large number of typical DC was proliferated by supplementing with GM-CSF and IL-4 cytokines. DC had specific cell appearance and structure, and highly expressed various cell surface molecules. TNF-? had the ability of stimulating DC mature, the mature DC had the enhancing abilities of antigen presenting and IL-12 self-secreting, as well as, expressed higher levels of CD54, MHC-Ⅱ and CD86 molecules than control group (P
