Detection of p53 Gene Mutation in Endemic Arsenism Patients by PCR-SSCP
- VernacularTitle:聚合酶链反应-单链构象多态法在研究新疆地方性砷中毒患者p53基因突变中的应用
- Author:
Huifang XIE
;
Yujian ZHENG
;
Yuexin ZHANG
- Publication Type:Journal Article
- Keywords:
Arsenic poisoning;
Mutation;
p53 gene
- From:
Journal of Environment and Health
1989;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study whether arsenic can induce p53 gene mutation in the lymphocytes in the peripheral blood and the skin cancer tissue of the patients with endemic arsenism in Xinjiang. Methods PCR-SSCP silver staining technique was employed to detect the mutation of exon E5-9 of p53 gene. Results Gel electrophoresis imaging demonstrated Exon E6, 7 of p53 gene appeared abnormal strips, strip absence and strip shift. Conclusion Arsenic can induce p53 gene mutation, the mutation sites located on exon E6,7.
