Expression of interferon-stimulated gene ISG20 and investigation of its antiviral effect on HCV replicon
- VernacularTitle:干扰素刺激基因ISG20真核表达载体的构建及其抗丙型肝炎病毒复制作用的研究
- Author:
Yintang JIA
;
Lai WEI
;
Dong JIANG
;
Xu CONG
;
Ran FEI
- Publication Type:Journal Article
- Keywords:
Interferon;
ISG20;
Clone;
Expression;
Hepatitis C virus;
Replicon
- From:
Chinese Journal of Immunology
2000;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the antiviral effect of ISG20 on HCV replicon.Methods:Wild type ISG20/mutated ISG20 cDNAs were obtained by RT-PCR/two step-PCR directed mutagenesis, and wild type ISG20 and dominant negative mutated ISG20 mammal expression vectors were consuructed. The constructed pISG20wt and pISG20m expressing vectors were transfected into Huh7 cells or Huh7 cells containing HCV replicon to investigate its effects on HCV replicon replication.Results:The ISG20wt/ISG20m expression vectors were constructed and the expressions of these two vectors were confirmed at both mRNA and protein levels. The effects of ISG20wt on HCV replicon replication were evaluated by Northern blot and Western blot. The results showed that expression of ISG20wt had significant inhibitory effect on HCV RNA replication.Conclusion:ISG20 participates in the anti-HCV action of IFN-? on HCV replicon system.
