Study of blockading Stat3 by transcription factor decoy-ODN inhibiting the proliferation of breast cancer cell line MDA-MB-231
- VernacularTitle:应用“decoy”技术靶向性阻断Stat3对乳腺癌细胞系MDA-MB-231增殖抑制的研究
- Author:
Jianling BAO
;
Shufen WANG
;
Xulong ZHANG
;
Jian ZHANG
- Publication Type:Journal Article
- Keywords:
Breast cancer;
Decoy;
Stat;
Oligodeoxynucletides
- From:
Chinese Journal of Immunology
2000;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the mechanisms of decoy oligodeoxynucleotides(decoy-ODN) blockading Stat3 that inhibit breast cancer cells MDA-MB-231 proliferation.Methods:Stat3 decoy-ODN and scramble control were transfected into breast cell line MDA-MB-231, respectively. The cell proliferation capability was detected by cell counting; flow cytometry was applied to detect MDA-MB-231 cell cycle; FITC labeled decoy was observed by Reflected Light Fluorescence Microscope; the expression of the gene controlled by Stat3 was examined by means of RT-PCR and Western blot assay.Results:Stat3 decoy-ODN could be internalized into MDA-MB-231 cells and inhibit the growth of MDA-MB-231 cell via inducing its apoptosis. Stat3 decoy-ODN also could significantly reduce the expression of Stat3 controlling genes such as Bcl-xl,c-myc and CylinD1.Conclusion:Stat3 decoy-ODN can inhibit breast cell line MDA-MB-231 proliferation by blockading JAK/STAT pathway. This suggests that transcription factor decoy-ODN may serve as a novel therapeutic strategy for breast cancer.
