Vaccine preparation of dendritic cell transfected with HPV16E6 antigen gene and its biological characteristics
- VernacularTitle:转染HPV16E6基因人树突状细胞疫苗的制备及生物学特性
- Author:
Huijun REN
;
Jinkun ZHANG
;
Dongxiao CHEN
;
Jun LI
;
Xiyun WEI
- Publication Type:Journal Article
- Keywords:
Dendritic cell;
E6 gene;
Gene transfection;
Lipofectamine;
Caski cells
- From:
Chinese Journal of Immunology
2000;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To prepare the vaccine of DC derived from human peripheral blood and transfected with HPV16E6 antigen gene, and to detect its morphological character,surface marker and immunological effect.Methods:DC-enriched populations were prepared from human peripheral blood mononuclear cell(PBMC) with the combination of rhGM-CSF,rhIL-4 and rhTNF-?. The plasmid containing HPV16E6 gene was transfected into DC with lipofectamine. The morphology of DC was observed dynamically, and the expression of surface markers of DC vaccine could be detected using immuno-cytochemical staining and flow cytometry. MTT assay was applied to detect the activity of CTL in vitro.Results:The transfected DC had typical morphologic and phenotypic characteristics, and expressed E6 protein 47.3%, CD80 82.5%, CD86 79.8% and CD83 85.7%. The killing activities of CTL to Caski cells induced by transfected DC were higher evidently than that of control groups(P
