Osteogenic differentiation of adipose-derived stem cells from rabbits in vitro
- VernacularTitle:兔脂肪干细胞的分离培养鉴定及成骨诱导分化研究
- Author:
Wei HAO
;
Yunyu HU
;
Yiyong WEI
- Publication Type:Journal Article
- Keywords:
rabbit;
adipose-derived stem cells;
osieogenic differentiation;
bone tissue engineering
- From:
Orthopedic Journal of China
2006;0(21):-
- CountryChina
- Language:Chinese
-
Abstract:
[Objective]To observe the differentiation of adipose derived stem cells(ADSCs)into osteogenic cells in rabbit in vitro.[Method]ADSCs were prepared by collagenase Ⅰ digestion of subcutaneous fat from the nape of Japanese white rabbit after being excised and finely minced.Cells were identified by stro-1 immunocy to chemistry,and collagen Ⅰ immunocytochemistry,alkaline phosphatase assay and Von Kossa stain after osteogenic differentiation was performed.[Result](1)ADSCs were stro-1 positive.(2)The expression of collagen Ⅰ,alkaline phosphatase and calcium deposit of ADSCs were all positive under the influence of osteogenic differentiation medium.[Conclusion]ADSCs have similar characteristics to bone marrow stromal cells,but are much more easier to have high number upon harvest and bring less pain will occur during taking ADSCs than taking BMDCs.
